Effects of dilute acid and flowthrough pretreatments and BSA supplementation on enzymatic deconstruction of poplar by cellulase and xylanase.
Identifieur interne : 001415 ( Main/Exploration ); précédent : 001414; suivant : 001416Effects of dilute acid and flowthrough pretreatments and BSA supplementation on enzymatic deconstruction of poplar by cellulase and xylanase.
Auteurs : Samarthya Bhagia [États-Unis] ; Rajeev Kumar [États-Unis] ; Charles E. Wyman [États-Unis]Source :
- Carbohydrate polymers [ 1879-1344 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Cellulase, Cellulose, Endo-1,4-beta xylanases, Lignine.
- Bois, Hydrolyse, Populus, Sérumalbumine bovine.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cellulase, Cellulose, Endo-1,4-beta Xylanases, Lignin.
- Hydrolysis, Populus, Serum Albumin, Bovine, Wood.
Abstract
To help understand factors controlling the recalcitrance of lignocellulosic biomass to deconstruction to sugars, poplar was pretreated with liquid hot water (LHW) and extremely dilute acid (EDA) at 140°C and 180°C in batch and flowthrough reactors. The resulting solids were then subjected to enzymatic hydrolysis by eight combinations of cellulase, xylanase, and bovine serum albumin (BSA). Co-addition of xylanase to cellulase resulted in up to 11 percentage points higher overall sugar yield than their sequential addition. In general, supplementation of BSA to enzymes had a larger impact on flowthrough solids with reduced lignin content than batch solids with high lignin content. BSA did not affect xylan yields and while it had low impact on LHW solids, it caused large increases in sugar yields from EDA solids. Flowthrough pretreatment produced less recalcitrant solids than did batch operation, but using very dilute acid reduced recalcitrance even more.
DOI: 10.1016/j.carbpol.2016.11.085
PubMed: 27987914
Affiliations:
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Le document en format XML
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Electronic address: sbhag001@ucr.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: rkumar@cert.ucr.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: cewyman@engr.ucr.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author></analytic><series><title level="j">Carbohydrate polymers</title><idno type="eISSN">1879-1344</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulase (metabolism)</term><term>Cellulose (metabolism)</term><term>Endo-1,4-beta Xylanases (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Lignin (metabolism)</term><term>Populus (MeSH)</term><term>Serum Albumin, Bovine (MeSH)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bois (MeSH)</term><term>Cellulase (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Endo-1,4-beta xylanases (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Lignine (métabolisme)</term><term>Populus (MeSH)</term><term>Sérumalbumine bovine (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Cellulose</term><term>Endo-1,4-beta Xylanases</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Cellulose</term><term>Endo-1,4-beta xylanases</term><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrolysis</term><term>Populus</term><term>Serum Albumin, Bovine</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bois</term><term>Hydrolyse</term><term>Populus</term><term>Sérumalbumine bovine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To help understand factors controlling the recalcitrance of lignocellulosic biomass to deconstruction to sugars, poplar was pretreated with liquid hot water (LHW) and extremely dilute acid (EDA) at 140°C and 180°C in batch and flowthrough reactors. The resulting solids were then subjected to enzymatic hydrolysis by eight combinations of cellulase, xylanase, and bovine serum albumin (BSA). Co-addition of xylanase to cellulase resulted in up to 11 percentage points higher overall sugar yield than their sequential addition. In general, supplementation of BSA to enzymes had a larger impact on flowthrough solids with reduced lignin content than batch solids with high lignin content. BSA did not affect xylan yields and while it had low impact on LHW solids, it caused large increases in sugar yields from EDA solids. Flowthrough pretreatment produced less recalcitrant solids than did batch operation, but using very dilute acid reduced recalcitrance even more.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">27987914</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1344</ISSN><JournalIssue CitedMedium="Internet"><Volume>157</Volume><PubDate><Year>2017</Year><Month>Feb</Month><Day>10</Day></PubDate></JournalIssue><Title>Carbohydrate polymers</Title><ISOAbbreviation>Carbohydr Polym</ISOAbbreviation></Journal><ArticleTitle>Effects of dilute acid and flowthrough pretreatments and BSA supplementation on enzymatic deconstruction of poplar by cellulase and xylanase.</ArticleTitle><Pagination><MedlinePgn>1940-1948</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0144-8617(16)31359-5</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.carbpol.2016.11.085</ELocationID><Abstract><AbstractText>To help understand factors controlling the recalcitrance of lignocellulosic biomass to deconstruction to sugars, poplar was pretreated with liquid hot water (LHW) and extremely dilute acid (EDA) at 140°C and 180°C in batch and flowthrough reactors. The resulting solids were then subjected to enzymatic hydrolysis by eight combinations of cellulase, xylanase, and bovine serum albumin (BSA). Co-addition of xylanase to cellulase resulted in up to 11 percentage points higher overall sugar yield than their sequential addition. In general, supplementation of BSA to enzymes had a larger impact on flowthrough solids with reduced lignin content than batch solids with high lignin content. BSA did not affect xylan yields and while it had low impact on LHW solids, it caused large increases in sugar yields from EDA solids. Flowthrough pretreatment produced less recalcitrant solids than did batch operation, but using very dilute acid reduced recalcitrance even more.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bhagia</LastName><ForeName>Samarthya</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: sbhag001@ucr.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: rkumar@cert.ucr.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: cewyman@engr.ucr.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>11</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Carbohydr Polym</MedlineTA><NlmUniqueID>8307156</NlmUniqueID><ISSNLinking>0144-8617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>27432CM55Q</RegistryNumber><NameOfSubstance UI="D012710">Serum Albumin, Bovine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.8</RegistryNumber><NameOfSubstance UI="D043364">Endo-1,4-beta Xylanases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043364" MajorTopicYN="N">Endo-1,4-beta Xylanases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012710" MajorTopicYN="N">Serum Albumin, Bovine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bovine serum albumin</Keyword><Keyword MajorTopicYN="N">Cellulase</Keyword><Keyword MajorTopicYN="N">Dilute acid</Keyword><Keyword MajorTopicYN="N">Flowthrough pretreatment</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Xylanase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>11</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27987914</ArticleId><ArticleId IdType="pii">S0144-8617(16)31359-5</ArticleId><ArticleId IdType="doi">10.1016/j.carbpol.2016.11.085</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Bhagia, Samarthya" sort="Bhagia, Samarthya" uniqKey="Bhagia S" first="Samarthya" last="Bhagia">Samarthya Bhagia</name></region><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. 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